Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro

BACKGROUND@#To explore the osteogenic and angiogenic potential of human vascular endothelial growth factor 165 (hVEGF165) gene-transfected canine bone marrow mesenchymal stem cells (BMSCs) combined with coral hydroxyapatite (CHA) scaffold. @*METHODS@#We constructed a lentiviral vector and transfected canine BMSCs with the best multiplicity of infection. Osteogenesis was induced in the transfected groups (GFP-BMSCs group and hVEGF-BMSCs group) and non-transfected group (BMSCs group), followed by the evaluation of alkaline phosphatase (ALP) activity and alizarin red S staining. Cells from the three groups were co-cultured with CHA granules, respectively to obtain the tissue-engineered bone. MTT assay and fluorescence microscopy were employed to assess cell proliferation and adhesion. The expression of osteogenic and angiogenic related genes and proteins were evaluated at 7, 14, 21, and 28 days post osteoinduction in cell culture alone and cell co-culture with CHA, respectively using RT-PCR and ELISA. @*RESULTS@#The hVEGF165 gene was transfected into BMSCs successfully. Higher ALP activity and more calcified nodules were found in the hVEGF-BMSCs group than in the control groups (p < 0.001). Cells attached and proliferated in CHA particles. Both cells cultured alone and cells co-culture with CHA expressed more osteogenic and angiogenic related genes and proteins in the hVEGF-BMSCs group compared to the GFP-BMSCs and BMSCs groups (p < 0.05). @*CONCLUSION@#High expression of hVEGF165 in BMSCs potentially promote the osteogenic potential of BMSCs, and synergically drive the expression of other osteogenic and angiogenic factors. hVEGF-BMSCs co-cultured with CHA expressed more osteogenic and angiogenic related factors, creating a favorable microenvironment for osteogenesis and angiogenesis. Also, the findings have allowed for the construction of a CHA-hVEGF-BMSCs tissue-engineered bone.

Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro

BACKGROUND@#To explore the osteogenic and angiogenic potential of human vascular endothelial growth factor 165 (hVEGF165) gene-transfected canine bone marrow mesenchymal stem cells (BMSCs) combined with coral hydroxyapatite (CHA) scaffold. @*METHODS@#We constructed a lentiviral vector and transfected canine BMSCs with the best multiplicity of infection. Osteogenesis was induced in the transfected groups (GFP-BMSCs group and hVEGF-BMSCs group) and non-transfected group (BMSCs group), followed by the evaluation of alkaline phosphatase (ALP) activity and alizarin red S staining. Cells from the three groups were co-cultured with CHA granules, respectively to obtain the tissue-engineered bone. MTT assay and fluorescence microscopy were employed to assess cell proliferation and adhesion. The expression of osteogenic and angiogenic related genes and proteins were evaluated at 7, 14, 21, and 28 days post osteoinduction in cell culture alone and cell co-culture with CHA, respectively using RT-PCR and ELISA. @*RESULTS@#The hVEGF165 gene was transfected into BMSCs successfully. Higher ALP activity and more calcified nodules were found in the hVEGF-BMSCs group than in the control groups (p < 0.001). Cells attached and proliferated in CHA particles. Both cells cultured alone and cells co-culture with CHA expressed more osteogenic and angiogenic related genes and proteins in the hVEGF-BMSCs group compared to the GFP-BMSCs and BMSCs groups (p < 0.05). @*CONCLUSION@#High expression of hVEGF165 in BMSCs potentially promote the osteogenic potential of BMSCs, and synergically drive the expression of other osteogenic and angiogenic factors. hVEGF-BMSCs co-cultured with CHA expressed more osteogenic and angiogenic related factors, creating a favorable microenvironment for osteogenesis and angiogenesis. Also, the findings have allowed for the construction of a CHA-hVEGF-BMSCs tissue-engineered bone.

Application of digital positioning guide plate in extraction of impacted supernumerary teeth / 南方医科大学学报

OBJECTIVE@#To explore the application of digital positioning guide plate in extraction of impacted supernumerary teeth and evaluate its clinical efficacy.@*METHODS@#From March to August, 2019, 30 patients with labial impacted supernumerary teeth treated in the Department of Oral and Maxillofacial Surgery, Stomatological Hospital, Southern Medical University were enrolled in this study. Fifteen of the patients were treated with digital positioning guide plate to remove the impacted supernumerary teeth (test group). According to the CBCT data and the dentition model of the patients, the positioning guide plate was positioned by avoiding the important anatomical structure with the tooth-bone surface as the support to design the soft tissue incision line and bone tissue exposure range. The other 15 patients who were treated without the use of the guide plate for operation served as the control group. The design time, tooth searching time, operation time and complications were compared between the two groups.@*RESULTS@#The positioning guide plate was well attached during the operation and allowed quick location of the supernumerary teeth while helping to expose the supernumerary teeth and avoid the damage of the adjacent important anatomical structures. The pre-operative design time was 50 ± 5 min in the test group and 0 min in the control group. The average time of tooth finding in the test group was 5±2 min, as compared with 10±3 min in the control group (=15.40, < 0.01); the average time of operation was significantly shorter in the test group than in the control group (25±4 min 45±6 min; =35.50, < 0.01). No intraoperative complications occurred in the test group, and slight deviation occurred in one case in the control group.@*CONCLUSIONS@#The application of digital positioning guide plate in extraction of embedded supernumerary teeth can significantly shorten the time of tooth finding, reduce the difficulty of operation, and improve the quality of operation.

Study on the mechanism of miR-497-induced laryngeal squamous cell carcinoma growth inhibition by targeting CDK6 / 中华耳鼻咽喉头颈外科杂志

Objective@#To study the effects of miR-497 and CDK6 on the growth of laryngeal squamous cell carcinoma (LSCC).@*Methods@#The expressions of CDK6 mRNA in fresh LSCC specimens, the adjacent normal mucosa of LSCC, and cell lines of LSCC were detected with quantitative real time polymerase chain reaction, pcDNA3.1(+) CDK6 plasmids were respectively transfected into the LSCC cells, and MTT assay and clone formation assay were performed to evaluate the growth of LSCC cells. Flow cytometry was employed for cell cycle analysis. SPSS17.0 software was used to analyze the data.@*Results@#CDK6 was highly expressed in LSCC(t=14.01, P=0.009) and the overall survival rate of the patients with high CDK6 expression was less than that with low CDK6 expression, with a significant difference (HR=3.236, P<0.001). Double luciferase reporter gene analysis showed that fluorescence activity in wild type CDK6 group was significantly different from that in control group (P<0.01), while there was no significant difference in the fluorescence activity between mutant CDK6 group and control group (P>0.05). A490 values were respectively 0.42±0.14 (Mean±SD) in siRNA Hep-2 group, 0.51±0.13 in siRNA TU-212 group; 0.98±0.16 in control Hep-2 group and 1.17±0.20 in control TU-212 group. Colonies were 55±4 in siRNA Hep-2 group, 51±3 in siRNA TU-212 group, 108±6 in control Hep-2 group and 105±7 in control TU-212 group, namely, cell growth and clone formation ability in CDK6 siRNA group were significantly lower than those in the control group. Cells cycle was blocked in G0/G1 phase (G0/G1: 65.20%±10.12% in siRNA Hep-2 group; 63.42%±8.97% in siRNA TU-212 group; 45.31%±7.55% in control Hep-2 group; and 42.37%±7.28% in control TU-212 group), and cells decreased obviously in S phase (S: 25.39%±5.51% in siRNA Hep-2 group; 27.21%±5.43% in siRNA TU-212 group; 42.87%±6.85% in control Hep-2 group; and 44.76%±7.02% in control TU-212 group). Compared with miR-497 group, cell growth and clone formation ability in miR-497/CDK6 group were partly restored (all P<0.05).@*Conclusions@#CDK6 expression in LSCC is upregulated, functioning as an oncogene. High expression of CDK6 is a predictor for poor prognosis. miR-497, functioning as a tumor suppressor gene, inhibits the growth of LSCC by targeting CDK6.

Spinal Cord Stimulation for Pain Treatment After Spinal Cord Injury / 神经科学通报·英文版

In addition to restoration of bladder, bowel, and motor functions, alleviating the accompanying debilitating pain is equally important for improving the quality of life of patients with spinal cord injury (SCI). Currently, however, the treatment of chronic pain after SCI remains a largely unmet need. Electrical spinal cord stimulation (SCS) has been used to manage a variety of chronic pain conditions that are refractory to pharmacotherapy. Yet, its efficacy, benefit profiles, and mechanisms of action in SCI pain remain elusive, due to limited research, methodological weaknesses in previous clinical studies, and a lack of mechanistic exploration of SCS for SCI pain control. We aim to review recent studies and outline the therapeutic potential of different SCS paradigms for traumatic SCI pain. We begin with an overview of its manifestations, classification, potential underlying etiology, and current challenges for its treatment. The clinical evidence for using SCS in SCI pain is then reviewed. Finally, future perspectives of pre-clinical research and clinical study of SCS for SCI pain treatment are discussed.

3D printed external fixator for treatment of tibial fractures / 中华骨科杂志

Objective To investigate the feasibility and primary clinical outcomes of a kind of independent research and development customized 3D printed external fixator in the treatment of tibial fracture.Methods Data of 7 tibial fracture patients who were treated using computer-assisted reduction and 3D printing technique including a new kind of customized external fixator with automatical reduction function from December 2013 to November 2015 were retrospectively analyzed.There were 5 males and 2 females in this cohort.The age of the patients ranged from 25 to 49 years old,with an average age of 38.1 years old.The procedure was as follows.At first,two or three pins were placed separately into the distal and proximal tibia away from fracture site.Then,the CT scanning was performed on the fractured tibia.According to the CT scanning data,the external fixator was designed by computer software and manufactured by the 3D printing technique.The reduction of tibial fracture was achieved automatically after the 3D printed external fixator was connected to the inserted pins.Finally,the accuracy of reduction was assessed by postoperative X-ray image.During the different fracture healing course,the stress environment could be dynamically adjusted by screwing or unscrewing the nuts to fulfill rigid fixation,compression fixation or elastic fixation for the bone healing.Results All the 7 cases had obtained successful reduction by only one time operation with the 3D printed external fixators.The average lateral angulation was 1.42°± 1.13°,and the average anteroposterior angulation was 1.65°± 1.36°,while the average lateral displacement was 1.38± 1.44 mm,and the average anteroposterior displacement was 1.83± 1.30 mm,which were measured from postoperative X-ray image.The individual durations of the operation were 9 min,8 min,9 min,1.5 min,1.4 min,1.4 min and 2.4 min,respectively.All fractures had achieved bony union in 19-25 weeks after operation,with an average time of 21.4±1.6 weeks.All patients were followed up for 6 months when removal of the external fixator,and no refracture occurred.Conclusion The customized 3D printed external fixator has the function of automatical reduction.In addition,it can provide three kinds of fixation modes,which are rigid fixation,compression fixation and elastic fixation.It has the advantages of simple operation,accurate reduction,reasonable fixation,etc.

Incidence and risk factors of intraoperative deep venous thrombosis in patients with multiple injuries / 中华创伤骨科杂志

Objective To investigate the incidence and risk factors of intraoperative deep venous thrombosis (DVT) in patients with multiple injuries.Methods A retrospective analysis was conducted of the 183 patients with multiple injuries who had been admitted to the Department of Orthopaedics and Trauma,Xi'an Honghui Hospital from January 2016 to November 2017.They were 117 males and 66 females,aged from 16 to 84 years (average,47.3 years).Craniocerebral injuries occurred in 16 cases,chest injuries in 24 cases,spinal injuries in 9 cases,pelvic acetabular fractures in 64 cases,upper limb fractures in 40 cases,and lower limb fractures in 145 cases.Venous ultrasonography of bilateral lower limbs was performed preoperatively and postoperatively to detect incidence of DVT.The patients were divided into a DVT group and a non-DVT group.The 2 groups were compared in terms of gender,age,interval from injury to surgery,D-dimer levels upon admission and discharge,operation time,intraoperative blood transfusion,injury severity scoring (ISS) and type of injury.After single-factor analysis was used to screen risk factors,multivariate logistic regression analysis was used to determine the main independent risk factors.Results The incidence of lower limb DVT in patients with multiple injuries was 42.08％ (77/183).Of the 77 DVT patients,12 had proximal thrombosis (6.56％) and 65 distal thrombosis (35.52％).Multivariate logistic regression analysis showed that age of ＞ 60 years,operative time for ≥2 hours,and presence of spinal injury were independent risk factors for DVT in patients with multiple injuries (P ＜ 0.05).Conclusions The incidence of lower limb DVT can be high in patients with multiple injuries.An age of ＞ 60 years,operation time for ≥2 hours and presence of spinal injury are independent risk factors for DVT in these patients.

Clinical characteristics and risk factors of proximal deep venous thrombosis in patients with fractures / 中华创伤杂志

Objective To investigate the clinical characteristics and risk factors of perioperative deep venous thrombosis (DVT) in fracture patients.Methods A retrospective case control study was conducted to analyze the clinical data of 147 patients with DVT diagnosed by color Doppler ultrasound or lower extremity deep venography from July 2015 to November 2017 in Honghui Hospital Affiliated to Xi'an Jiaotong University College of Medicine.The patients were divided into proximal thrombosis group (including the popliteal vein) and distal thrombosis group according to the involvement site with the popliteal vein as reference.The proximal thrombosis group (56 patients) included 31 males and 25 females,aged 38-89 years,with an average age of 67.8 years.There were three patients with pelvic and acetabular fractures,39 with hip fractures,10 with femoral shaft fractures,three with periarticular fractures,and one with tibia and fibula fracture.According to the site of involvement,four patients had proximal vein thrombosis,and the other 52 patients had proximal combined with distal thrombosis.There were 44 patients with left thrombosis and 12 with right thrombosis.Thrombosis at femoral vein and above was found in 20 patients (7 with external iliac vein thrombosis),and thrombosis at popliteal vein was found in 36 patients.The distal thrombosis group (91 patients) consisted of 51 males and 40 females,aged 21-92 years,with an average of 63.6 years.There were 12 patients with pelvic and acetabular fractures,39 with hip fractures,four with femoral shaft fractures,16 with fractures surrounding knee joint,12 with tibia and fibula fractures,and eight with ankle joint fractures.There were 43 patients with left thrombosis and 48 with right thrombosis.The gender,age,fracture location,time from injury to operation,operation time,blood transfusion rate,thrombus side,D-dimer level before and after operation,and internal medical diseases were compared between the two groups.The risk factors with statistical significance were screened for multivariate Logistic regression analysis.Results In proximal thrombosis group,70％ (39/56) had hip fracture and 18％ (10/56) had femoral shaft fracture,while 43％ (39/91) had hip fracture and 4％ (4/91) had femoral shaft fracture in distal thrombosis group (P＜0.05).In the proximal thrombosis group,79％ (44/56) had thrombosis in the left lower extremity,which was much higher than that in the distal thrombosis group 47％ (43/91) (P ＜ 0.05).In proximal thrombosis group,77％ (43/56) received blood transfusion,which was higher than 39％ (35/91) in distal thrombosis group (P ＜0.05).D-dimer levels in proximal thrombosis group before and after operation [(8.5 ± 8.1) mg/L,(12.8 ± 9.7) mg/L],were higher than those in distal thrombosis group [(4.6 ± 3.8) mg/L,(7.2 ± 7.5) mg/L] (P ＜ 0.05).There were no significant differences in gender,age,time from injury to operation,operation time and internal medical complications between the two groups (P ＞0.05).Logistic regression analysis showed that hip fracture and femoral shaft fracture,left lower limb,blood transfusion,high D-dimer level before and after operation were risk factors for proximal thrombosis.Conclusions Proximal venous thrombosis is often combined with distal venous thrombosis.Hip fracture,femoral shaft fracture,left lower limb,blood transfusion,preoperative and postoperative high D-dimer levels have significant effects on proximal thrombosis.

The effect of miR-497 on laryngeal squamous cell carcinoma invasion through modulating PlexinA4 / 中华耳鼻咽喉头颈外科杂志

Objective@#To study the roles of miR-497 and PlexinA4 in the progression of laryngeal squamous cell carcinoma.@*Methods@#The expressions of miR-497 and PlexinA4 in fresh tumor specimens and adjacent normal mucosa tissues as well as in cell lines of laryngeal squamous cell carcinoma (LSCC) were detected with qRT-PCR and immunohistochemistry. The association of miR-497 and PlexinA4 expressions with clinicopathologic factors and their prognostic values in LSCC were evaluated PlexinA4 siRNA and pcDNA3.1 (+ )/PlexinA4 plasmid were transfected into the LSCC and measured by Transwell to evaluate their effect on the invasion of LSCC.@*Results@#miR-497 was low expression in LSCC, which related to pathological differentiation, while PlexinA4 mRNA was high expression in LSCC. Kaplan-Meier method showed that the prognosis of patients with high miR-497 expression was better than that of patients with low miR-497 expression (χ2=10.342, P=0.001); . Cox regression analysis showed that miR-497 was an independent prognostic factor for LSCC. The double luciferase reporter gene showed that the variation of the fluorescence activity of wild type PlexinA4 was significantly different from that of the control group (P<0.01). In Hep-2 and TU212 cell line, the number of cells with PlexinA4 siRNA passing through the compartments was 70.00±10.85 and 85.00±6.45, significantly higher than control (F values were 30.251 and 23.936, both P<0.05), the number of cells with pcDNA3.1 (+ ) /PlexinA4 was 170.56±11.95 and 142.00±10.43, also significantly less than control (F values were 35.104 and 29.643, both P<0.05).@*Conclusion@#The expression of miR-497 in LSCC is decreased, indicating poor prognosis, which is as an independent risk factor for prognosis of LSCC. miR-497 may modulate LSCC invasion through PlexinA4.

Tranexamic acid reduces hidden blood loss in treatment of intertrochanteric fractures with proximal femoral nail anti-rotation / 中华创伤骨科杂志

Objective To assess the effect of preoperative administration of tranexamic acid (TXA) on the hidden blood loss in the surgery of intertrochanteric fractures with proximal femoral nail anti-rotation (PFNA).Methods Eighty patients with intertrochanteric fracture were treated with PFNA in our hospital from November 2015 to July 2016.They were 15 men and 65 women,with a mean age of 72.6 years.Of them,39 were included into TXA group where TXA was administered preoperatively and 41 were assigned into the control group where no TXA was used preoperatively.Blood routine examinations were carried out on one day before operation,the first and third days after operation.The surgical blood loss,operative blood transfusion,24-hour drainage after operation,and postoperative 3-day blood transfusion were recorded.The total and hidden blood losses were calculated according to the formula.The 2 groups were compared in terms of blood loss and complications.Results In TXA group,the total blood loss (1,632.3 ± 849.2 mL),the hidden blood loss (1,270.9 ± 623.3 mL) and the transfusion rate (28.2％) were significantly lower than those in the control group (2,014.8 ± 924.7 mL,1,549.1 ± 624.9 mL and 56.1％) (P ＜ 0.05).There was no significant difference between the 2 groups in visible blood loss (361.4 ± 154.3 mL for TXA group versus 465.7 ± 191.3 mL for the control group) (P ＞ 0.05).Deep venous thrombosis occurred in 2 patients,limb swelling in one patient and wound dehiscence in one patient in TXA group while deep venous thrombosis occurred in one patient,limb swelling in 3 patients and wound dehiscence in none in the control group,showing no significant differences between the 2 groups (P ＞ 0.05).No infection was observed in either group.Conclusion Preoperative administration of TXA can reduce surgical hidden blood loss and transfusion rate as well but will not increase the risk of deep vein thrombosis in the surgery of intertrochanteric fractures with PFNA.

The expression of CD147 and PTEN in non small cell lung cancer and their correlation with clinical pathological factors / 重庆医学

Objective To analyze the expression of extracellular matrix metalloproteinase inducer (CD147)and phosphatase and tensin homolog deleted on chromosome ten(PTEN)in non small cell lung cancer (NSCLC) ,and to explore the correlations be‐tween expressions of CD147 and PTEN and those with clinicopathological factors .Methods The expressions of CD147 and PTEN proteins in tissues of 64 cases of patients with NSCLC and 10 cases of normal paracancerous tissues were determined by using im‐munohistochemical SP method .The correlations between expressions of CD147 and PTEN with clinicopathological factors were ana‐lysed ,as well .Results The expression of CD147 in NSCLC tissues(75 .00% )was significantly higher than that in paracancerous tissues(0 .00% ,P<0 .05) .The expression of CD147 was strongly associated with degrees of differentiation ,lymph node metastasis and TNM stage(P<0 .05) .The expression of PTEN in NSCLC tissues (32 .81% )was significantly lower than that in paracancerous tissues(80 .00% ,P<0 .05) .Expression of PTEN was strongly associated with TNM stage (P<0 .05) .Spearman correlation analy‐sis shown that CD147 expression was negatively correlated with PTEN expression (r= -0 .442 ,P<0 .05) .Conclusion The abnor‐mal expression of CD147 and PTEN might play an important role in the malignant progression of NSCLC .

Clinical application of heterogeneous acellular dermal matrix used in alveolar bone grafting / 中国医师进修杂志

Objective To observe the effect of bone repair and evaluate its esthetic outcome with heterogeneous acellular dermal matrix cover the alveolar cleft bone grafting area in the alveolar cleft operation.Methods In 67 cases,unilateral cleft palate,were treated by alveolar cleft conventional surgical method.Cancellous iliac bone grafting were control group,heterogeneous acellular dermal matrix cover the alveolar cleft bone grafting area were treatment group.Radiographs was taken at 1 st,3 rd,6 th,12 th,18 th,24 th month postoperatively to observe the bone regeneration alveolar cleft zone.Results The alveolar cleft graft area new bone formation with Ⅰ,Ⅱ,Ⅲ,Ⅳ grade after 6 months in control group was 15,11,9,6 cases and in treatment group was 13,9,3,1 case.The graft survival rate and success rate (97.8％,84.3％) of treatment group were higher than that of control group (84.5％,63.7％),the difference was statistically significant (P ＜ 0.05).Conclusion The successful rate of operation could be warranted,by the joint application of autogenous iliac bone grafts and heterogeneous acellular dermal matrix in the alveolar cleft operation.

Clinical Study of Different Dosage Atorvastatin in Treatment of Patients with Unstable Angina post Percutaneous Coronary Intervention / 医学研究杂志

Objective To investigate the effects of different dosage of atorvastation on inflammatory factors in patients with unstable angina(UAP)post PCI.Methods 116 patients with their coming order were randomly divided into two groups.The observation group of 56 cases with the dosage of 20mg/d,and the control group of 60 cases with the common dosage of 10mg/d.Blood samples were examined at the day of the operation preoperatively,24 h after PCI,4W,12W after administration.Results(1)The consistency of hs-CRP and cTnI of patients after PCI were more higher than that before PCI(P

The clinical application and evaluation of combined chemotherapy in comprehensive treatment for oral squamous cell carcinoma / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study and evaluate the clinical effects of combined preoperative chemotherapy and their relations with multi drug resistance (MDR).</p><p><b>METHODS</b>102 cases with oral squamous cell carcinoma(OSCC) were included in the study (63 males and 39 females, aged 22 to 67 years). Among the subjects there were 57 cases with cancer of tongue and 45 cases with cancer of buccal mucosa. 27 cases in the group were classified as stage II, 55 as stage III and 20 cases as stage IV according to TNM standard. All cases accepted PYM + 5-Fu + DDP combined chemotherapy pre-operatively. The total given dose was PYM 48 mg, 5-Fu 7.5 g and DDP 300 mg. After the chemotherapy, radical surgery were performed within 2 weeks. The diagnosis of all cases were proved as OSCC by biopsy.</p><p><b>RESULTS</b>Total effective rate of the combined chemotherapy was 82.4%. All of the cases were followed up and their 3 years' survival rate was 67.6%.</p><p><b>CONCLUSION</b>The combined chemotherapy of PYM + 5-Fu + DDP is effective in using as one of comprehensive treatment for OSCC.</p>

Clinical characters, diagnosis and treatment of parapharyngeal region neoplasms: a report of 115 cases / 华西口腔医学杂志

<p><b>OBJECTIVE</b>The aim of this study was to analyze the clinical characters, diagnosis and treatment of primary neoplasms in the parapharyngeal regions.</p><p><b>METHODS</b>A total of 115 cases of primary neoplasms in the parapharyngeal regions were included in this study.</p><p><b>RESULTS</b>Disorders of swallowing, breath or speaking were the common symptoms of neoplasms in parapharyngeal regions. The majority of neoplasms parapharyngeal regions were benign. 46 cases were from the deep lobe of the parotid, and 42 cases were neurogenic. CT or MRI provides useful information such as their sizes, shapes and extents. The relationship between the tumor and vessel was shown using super-selective angiography. All the tumors were resected completely by extra-oral approach through the posteroinferior incision of the mandible. The follow-up was carried out from 1 to 10 years, and the data indicated that 2 patients died, 18 cases were lost, and others had no recurrence.</p><p><b>CONCLUSION</b>CT and/or MRI are essentially useful for diagnosis and presurgical planning. Super-selective angiography can be used if the lesions involved carotid artery. Posteroinferior incision of the mandible is a simple, effective and safe approach for excision of neoplasms parapharyngeal regions.</p>

p53 mutation and microsatellite alteration in T cell lymphoma:38 cases / 第三军医大学学报

0.05). Conclusion There are MA and mutation of p53 gene in T-cell lymphoma though no significant correlation between them. But, MA positive cases might experience high mutation of p53 gene in T-cell lymphoma.